| Expression pattern: |
UP |
| Associated gene: |
KDM7A |
| Associated microRNA: |
miR-1258 |
| Biological function: |
Promotes breast cancer cell growth, invasion and migration, and maintains cancer stem-like cell properties (microsphere formation, stemness markers, ALDH1 activity); circ_002178 knockdown inhibits tumor growth and metastasis in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge binding miR-1258, thereby reducing miR-1258 inhibitory effect on KDM7A and promoting stem-like characteristics and malignant phenotypes. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
High expression of circ_002178 was correlated with the low survival rate, clinical tumor size, lymph node metastasis, and TNM stage/grade. |
| Description: |
hsa_circ_002178 is up-regulated in breast cancer tissues and cell lines and is associated with worse survival and aggressive clinicopathological features. Functionally, it promotes proliferation, invasion/migration and cancer stem-like properties. Mechanistically, it sponges miR-1258 to relieve repression of KDM7A, supporting tumor growth and metastasis in vitro and in a xenograft model. |
| Confidence score: |
0.8239 |