circRNA basic information
circBase ID: hsa_circ_0008450
Name: hsa_circ_CMTM3
Synonym: circ_0008450
Host Gene: CMTM3
Genomic location(hg19): chr16:66642211-66643906:+
Genomic location(hg38): chr16:66608308-66610003:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular cancer
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; serum; tumor tissues

Cell lines:

SNU-387; Huh7; THLE-2; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: AKAP1
Associated microRNA: miR-431
Biological function: Promotes HCC progression under hypoxia by increasing viability and glycolysis and inhibiting apoptosis; promotes tumor growth in vivo.
Molecular mechanism: Acts as a ceRNA sponge for miR-431 to upregulate AKAP1.
Biological pathway or process:

glycolysis (promotes); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis

Clinical significance:

circ_0008450 exhibited extremely high diagnostic value to distinguish patients with HCC from healthy controls (AUC=0.97). High circ_0008450 expression was associated with tumor size, TNM stage, lymphatic metastasis and distant metastasis.

Description:

hsa_circ_0008450 (circ_0008450), derived from CMTM3, is up-regulated in HCC tissues/serum and in hypoxia-treated HCC cells. It promotes hypoxia-driven malignant phenotypes (higher viability and glycolysis, lower apoptosis) and tumor growth by sponging miR-431 to increase AKAP1 expression, forming a circ_0008450/miR-431/AKAP1 axis with diagnostic potential.

Confidence score:

0.8158

Other information
Title:

Hypoxia-induced circular RNA hsa_circ_0008450 accelerates hepatocellular cancer progression via the miR-431/AKAP1 axis.

Journal: Oncology letters
Published: 2020
PubMed ID: 33193848
Study type:

combined biological and clinical study

Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request
Code availability: -