| Expression pattern: |
UP |
| Associated gene: |
AKAP1 |
| Associated microRNA: |
miR-431 |
| Biological function: |
Promotes HCC progression under hypoxia by increasing viability and glycolysis and inhibiting apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-431 to upregulate AKAP1. |
| Biological pathway or process: |
glycolysis (promotes); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0008450 exhibited extremely high diagnostic value to distinguish patients with HCC from healthy controls (AUC=0.97). High circ_0008450 expression was associated with tumor size, TNM stage, lymphatic metastasis and distant metastasis. |
| Description: |
hsa_circ_0008450 (circ_0008450), derived from CMTM3, is up-regulated in HCC tissues/serum and in hypoxia-treated HCC cells. It promotes hypoxia-driven malignant phenotypes (higher viability and glycolysis, lower apoptosis) and tumor growth by sponging miR-431 to increase AKAP1 expression, forming a circ_0008450/miR-431/AKAP1 axis with diagnostic potential. |
| Confidence score: |
0.8158 |