| Expression pattern: |
DN |
| Associated gene: |
FOXO1, IGF2BP3, ITGA5 mRNA, IGF2BP1, IGF2BP2 |
| Associated microRNA: |
miR-186-5p |
| Biological function: |
circEIF4B promotes osteogenic differentiation and bone regeneration in high glucose-mediated BMSCs; circEIF4B knockdown attenuates PA-induced osteogenesis and bone formation in diabetic rat cranial defects. |
| Molecular mechanism: |
circEIF4B acts as a ceRNA by sponging miR-186-5p to activate FOXO1; it also interacts with IGF2BP3, inhibits ubiquitin-mediated IGF2BP3 degradation, and stabilizes ITGA5 mRNA to promote osteogenic differentiation. |
| Biological pathway or process: |
ceRNA regulation (promotes); mRNA stability (promotes); ubiquitination (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; EdU Staining; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circEIF4B is proposed as a therapeutic target for high glucose-mediated BMSC osteogenic dysfunction and delayed bone regeneration in diabetes. |
| Description: |
In high-glucose BMSCs, circEIF4B is reduced, while PA treatment restores and upregulates its expression. circEIF4B promotes osteogenic differentiation and bone regeneration through two main mechanisms: sponging miR-186-5p to regulate FOXO1 and forming a complex with IGF2BP3 to stabilize ITGA5 mRNA. Knockdown of circEIF4B diminishes the osteogenic and bone-regenerative effects of PA in diabetic rat cranial defects. |
| Confidence score: |
0.8664 |