| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
hsa-miR-6833-3p, hsa-let-7i-3p, hsa-miR-1301-3p |
| Biological function: |
Potential non-invasive diagnostic biomarker for CRC (including early-stage CRC); may play a role in CRC pathogenesis. |
| Molecular mechanism: |
Predicted circRNA-miRNA-mRNA binding network; negative correlation with specific miRNAs (putative miRNA sponge/ceRNA role, not functionally validated). |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum exosomal circ-PNN distinguishes CRC from healthy controls (AUC 0.855 training; 0.826 validation) and has diagnostic value for early-stage CRC (AUC 0.854). |
| Description: |
Serum exosomal circ-PNN (hsa_circ_0101802) is up-regulated in CRC patients and shows good diagnostic performance (including early-stage CRC) by ROC analysis. The study builds a predicted circ-PNN-miRNA-mRNA network and reports negative correlations between circ-PNN and hsa-miR-6833-3p, hsa-let-7i-3p, and hsa-miR-1301-3p, suggesting a potential ceRNA-related role that requires further functional validation. |
| Confidence score: |
0.6841 |