circRNA basic information
circBase ID: hsa_circ_0005320
Name: hsa_circ_SEPT9
Synonym: circ_SEPT9
Host Gene: SEPT9
Genomic location(hg19): chr17:75398140-75398785:+
Genomic location(hg38): chr17:77402058-77402703:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004958
MONDO name: oral cavity squamous cell carcinoma
Disease details: oral squamous cell carcinoma
Disease DO ID:
 0050866
Disease MeSH ID:
 -
Disease NCIt ID:
 C4833
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OSCC tissue; adjacent normal tissue; tumour tissue; tumours of nude mice
Cell lines:

HN4; SCC-9; SCC-25; CAL-27; SCC-15; HSC-3; UM1; HOK
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PKN2
Associated microRNA: miR-1225
Biological function: Promotes OSCC cell proliferation, tumour growth, migration and invasion; circ_SEPT9 knockdown suppresses these phenotypes.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-1225, thereby rescuing/protecting PKN2 mRNA expression and increasing PKN2 protein.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Sanger Sequencing; Transfection; CCK8; Transwell Assay; Wound Healing Assay; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

circ_SEPT9/miR-1225/PKN2 axis is suggested as a promising therapeutic target for OSCC.
Description:

circ_SEPT9 is up-regulated in OSCC tissues and cell lines and promotes proliferation, tumour growth, migration and invasion. It functions as a miR-1225 sponge to relieve repression of PKN2, forming a circ_SEPT9/miR-1225/PKN2 ceRNA axis that drives OSCC progression.
Confidence score:

0.8115
Other information
Title:

circ_SEPT9, a newly identified circular RNA, promotes oral squamous cell carcinoma progression through miR-1225/PKN2 axis.

Journal: Journal of cellular and molecular medicine
Published: 2020
PubMed ID: 33090705
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -