| Expression pattern: |
DN |
| Associated gene: |
RET |
| Associated microRNA: |
miR-146b-5p |
| Biological function: |
Circ-ITCH overexpression promoted proliferation and migration of 293T and SH-SY5Y cells and activated the MAPK signaling pathway; circ-ITCH knockdown suppressed proliferation and migration. |
| Molecular mechanism: |
Circ-ITCH functions as a ceRNA by directly binding and negatively regulating miR-146b-5p, thereby relieving miR-146b-5p-mediated inhibition of RET and activating the RET/MAPK axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); MAPK (promotes); ERK (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
Circ-ITCH was downregulated in HSCR colon tissues and cells. Its overexpression promoted proliferation and migration of 293T and SH-SY5Y cells by sponging miR-146b-5p, increasing RET expression, and activating the MAPK/ERK pathway, suggesting a potential protective role against HSCR-related cellular defects. |
| Confidence score: |
0.6536 |