| Expression pattern: |
UP |
| Associated gene: |
VEGF, Ago2, ZFR |
| Associated microRNA: |
miRNA-377 |
| Biological function: |
promotes ESCC cell proliferation, migration, and invasion in vitro; promotes tumor growth/cell proliferation in vivo |
| Molecular mechanism: |
circ_0072088 acts as a miRNA sponge for miR-377 to upregulate VEGF expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Transwell Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
circ_0072088 overexpression was closely associated with tumor size, invasion depth, clinical stage, and lymph node metastasis; may be used as a diagnostic biomarker and therapeutic target |
| Description: |
circ_0072088 is upregulated in ESCC and promotes proliferation, migration, and invasion. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-377, thereby increasing VEGF expression and enhancing malignant phenotypes, including tumor growth in a xenograft model. |
| Confidence score: |
0.8558 |