| Expression pattern: |
UP |
| Associated gene: |
SEC31A |
| Associated microRNA: |
miR-376a, miR-376b, miR-383-5p, miR-525-5p |
| Biological function: |
promotes migration, invasion and glycolysis and inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-376a to regulate SEC31A expression |
| Biological pathway or process: |
migration (promotes); invasion (promotes); glycolysis (promotes); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; Transwell Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
circSEC31A expression was closely associated with tumor size, TNM stage and lymphatic metastasis |
| Description: |
circSEC31A is up-regulated in NSCLC tissues and cell lines and promotes malignant phenotypes (migration, invasion, glycolysis, and tumor growth) while inhibiting apoptosis. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-376a, thereby relieving miR-376a-mediated repression of SEC31A. |
| Confidence score: |
0.762 |