circRNA basic information
circBase ID: hsa_circ_0052586
Name: hsa_circ_RRM2
Synonym: circRRM2
Host Gene: RRM2
Genomic location(hg19): chr2:10268974-10271546:+
Genomic location(hg38): chr2:10128847-10131419:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0013209
MONDO name: metabolic dysfunction-associated steatotic liver disease
Disease details: metabolic dysfunction-associated steatotic liver disease
Disease DO ID:
 0080208
Disease MeSH ID:
 D065626
Disease NCIt ID:
 C84444
Disease ICD11 ID:
 1912806631
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

liver tissues; livers
Cell lines:

HepG2; Huh7
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: NRG1, Ago2, SREBP1, LXRa, FASN, SCD1, ACC1, CD36
Associated microRNA: miR-142-5p
Biological function: circRRM2 attenuates MASLD development, suppresses FFA-induced triglycerides accumulation, mitigates intracellular TG accumulation and lipid droplet formation, inhibits lipogenesis-related factors, and ameliorates HFD-induced steatosis and pathological changes.
Molecular mechanism: circRRM2 acts as a miR-142-5p sponge, suppresses miR-142-5p, upregulates the miR-142-5p target NRG1, and thereby inhibits lipogenesis and triglyceride accumulation in MASLD models.
Biological pathway or process:

lipid metabolism (inhibits); inflammation (inhibits); ceRNA regulation (other); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Oil Red O Staining; Western Blot; In Vivo Animal Model; H&E Staining; ELISA; Bioinformatics Analysis
Clinical significance:

-
Description:

circRRM2 is a human RRM2-derived circRNA that is downregulated in FFA-treated hepatocytes and HFD-induced MASLD mouse livers. Its overexpression alleviates MASLD-associated steatosis by sponging miR-142-5p, increasing NRG1 expression, and suppressing lipogenesis-related gene expression, triglyceride accumulation, lipid droplet formation, and inflammatory/pathological changes.
Confidence score:

0.7712
Other information
Title:

Circular RNA RRM2 alleviates metabolic dysfunction-associated steatotic liver disease by targeting miR-142-5p to increase NRG1 expression.

Journal: American journal of physiology. Gastrointestinal and liver physiology
Published: 2024
PubMed ID: 39259911
Study type:

combined biological and bioinformatics study
Data availability: GSE185062; GSE89632; Supplemental Tables S1 and S2: https://doi.org/10.6084/m9.figshare.25933966; Supplemental Figs. S1-S3: https://doi.org/10.6084/m9.figshare.25934200
Code availability: -