| Expression pattern: |
UP |
| Associated gene: |
USP33, c-Myc |
| Associated microRNA: |
hsa-miR-206 |
| Biological function: |
promotes prostate cancer cell proliferation and colony formation, promotes cell cycle transition, increases docetaxel resistance/suppresses sensitivity to docetaxel, and promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponge of miR-206, thereby positively regulating USP33, which deubiquitinates c-Myc and increases c-Myc protein |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes); drug resistance (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Western Blot; Co-IP; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Survival Analysis |
| Clinical significance: |
may serve as a novel biomarker for prostate cancer |
| Description: |
circ_0057558 is up-regulated in prostate cancer and acts oncogenically by promoting proliferation and cell-cycle progression. Mechanistically, it sponges miR-206 to upregulate USP33, which deubiquitinates and stabilizes c-Myc, and it reduces sensitivity to docetaxel. |
| Confidence score: |
0.805 |