| Expression pattern: |
UP |
| Associated gene: |
Ago2 |
| Associated microRNA: |
miR-622 |
| Biological function: |
Promotes CRC cell proliferation, migration and invasion, and inhibits apoptosis; counteracts the inhibitory effect of sevoflurane on CRC progression and tumor growth. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA-like) for miR-622; validated by luciferase reporter and Ago2-RIP. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; MTT; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Western Blot; Clinical Sample Validation; Survival Analysis; In Vivo Animal Model |
| Clinical significance: |
High hsa_circ_0000231 expression is associated with low survival rate (poor prognosis) in CRC patients. |
| Description: |
hsa_circ_0000231 is up-regulated in colorectal cancer tissues/cells and promotes malignant behaviors (proliferation, migration/invasion) while inhibiting apoptosis. Mechanistically, it sponges miR-622 (validated by luciferase and Ago2-RIP), and its overexpression counteracts sevoflurane-induced suppression of CRC progression and tumor growth; high expression predicts poorer survival. |
| Confidence score: |
0.7858 |