circRNA basic information
circBase ID: hsa_circ_0000467
Name: hsa_circ_SKA3
Synonym: circSKA3
Host Gene: SKA3
Genomic location(hg19): chr13:21742126-21742538:-
Genomic location(hg38): chr13:21167987-21168399:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; matched adjacent normal tissues; subcutaneous tumors
Cell lines:

HCT116; SW620; SW480; DLD1; CaCO2; HT29; RKO; NCM460
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: beta-catenin, CK1, GSK3beta, beta-TrCP1, Axin2, c-Myc, Cyclin D1
Associated microRNA: -
Biological function: Promotes CRC cell activity, viability, proliferation, migration and tumor growth, inhibits apoptosis, and counteracts the suppressive effects of sevoflurane on CRC malignancy.
Molecular mechanism: circSKA3 directly binds beta-catenin, disrupts beta-catenin interaction with the CK1/GSK3beta/beta-TrCP1 destruction complex, inhibits beta-catenin ubiquitinated proteasomal degradation, stabilizes beta-catenin protein, and activates Wnt/beta-catenin signaling; SEV suppresses this mechanism by reducing circSKA3 expression.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ubiquitination (inhibits); drug resistance (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; IF (Immunofluorescence); IHC (Immunohistochemistry); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circSKA3 expression was correlated with tumor T stage, lymph node status, distant metastasis, UICC grade, tumor size, histological grade, and poor overall survival.
Description:

circSKA3 is an upregulated oncogenic circRNA in CRC, and high expression is associated with poorer overall survival and advanced clinicopathological features. Mechanistically, circSKA3 binds beta-catenin, inhibits its destruction complex-mediated ubiquitinated degradation, activates Wnt/beta-catenin signaling, and promotes malignant CRC phenotypes; sevoflurane suppresses CRC partly by reducing circSKA3.
Confidence score:

0.8961
Other information
Title:

Sevoflurane suppresses colorectal cancer malignancy by modulating beta-catenin ubiquitination degradation via circSKA3.

Journal: Cellular signalling
Published: 2024
PubMed ID: 38029946
Study type:

combined biological and clinical study
Data availability: GSE126094; GSE138589; GSE442837; Supplementary data to this article can be found online at https://doi.org/10.1016/j.cellsig.2023.110987
Code availability: -