| Expression pattern: |
UP |
| Associated gene: |
CDCA4 / Cell Division Cycle-Associated protein 4 |
| Associated microRNA: |
miR-503-5p |
| Biological function: |
circ_0010220 promotes osteosarcoma cell proliferation, cell cycle progression, migration, invasion and in vivo tumor growth, and inhibits apoptosis; knockdown of circ_0010220 suppresses these malignant phenotypes and induces apoptosis and G1 cell-cycle arrest. |
| Molecular mechanism: |
circ_0010220 functions as a ceRNA/molecular sponge for miR-503-5p and regulates the downstream target CDCA4. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0010220 can be an important biomarker for osteosarcoma |
| Description: |
circ_0010220 is up-regulated in osteosarcoma cells and acts as an oncogenic circRNA. Its knockdown suppresses proliferation, migration, invasion and xenograft tumor growth, while inducing apoptosis and G1 cell-cycle arrest. Mechanistically, circ_0010220 sponges miR-503-5p to regulate CDCA4 expression, suggesting potential biomarker value in osteosarcoma. |
| Confidence score: |
0.6835 |