| Expression pattern: |
UP |
| Associated gene: |
IGFBP3, Ago2 |
| Associated microRNA: |
miR-409-3p |
| Biological function: |
promotes proliferation, migration and invasion and inhibits apoptosis of osteosarcoma cells |
| Molecular mechanism: |
circ_0000285 acts as a miRNA sponge for miR-409-3p to upregulate IGFBP3 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
The expression of circ_0000285 was positively associated with tumor size. |
| Description: |
circ_0000285 is up-regulated in osteosarcoma tissues and cell lines and mainly localizes to the cytoplasm. It promotes osteosarcoma cell proliferation, migration and invasion while inhibiting apoptosis by sponging miR-409-3p to upregulate IGFBP3, and its knockdown suppresses tumor growth in a xenograft model. |
| Confidence score: |
0.7984 |