| Expression pattern: |
UP |
| Associated gene: |
CCNE1 |
| Associated microRNA: |
miR-34a |
| Biological function: |
Promotes NSCLC cell viability/proliferation, migration, and invasion; inhibits apoptosis; facilitates cell cycle progression (reduces G0/G1 arrest). |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-34a to increase CCNE1 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); p53 signaling (other); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Clinical Sample Validation |
| Clinical significance: |
Proposed as a prognostic indicator and therapeutic target in NSCLC, but correlation with survival time was not established in this study. |
| Description: |
circDENND2A is up-regulated in NSCLC tissues and promotes malignant phenotypes (proliferation/viability, migration, invasion) while suppressing apoptosis and G0/G1 arrest. Mechanistically, it functions as a ceRNA that sponges miR-34a to increase CCNE1 expression, and CCNE1 restoration rescues the effects of circDENND2A knockdown. |
| Confidence score: |
0.6536 |