| Expression pattern: |
UP |
| Associated gene: |
PD-L1 |
| Associated microRNA: |
miR-214 |
| Biological function: |
Promotes CRC proliferation, invasion and tube formation/angiogenesis-like phenotypes and promotes xenograft tumor growth. |
| Molecular mechanism: |
CAF-derived exosomal circEIF3K acts as a miRNA sponge/ceRNA for miR-214, thereby relieving miR-214-mediated suppression of PD-L1 and promoting CRC progression under hypoxia. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); angiogenesis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Bioinformatics Analysis; Transfection; Colony Formation Assay; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
High circEIF3K level is associated with worse overall survival and higher tumor stage in CRC (TCGA). |
| Description: |
circEIF3K is enriched in CAF-derived exosomes under hypoxia and promotes CRC malignant phenotypes and xenograft growth. Mechanistically, circEIF3K binds miR-214, leading to increased PD-L1 expression and enhanced CRC progression; higher circEIF3K correlates with advanced stage and poorer survival in TCGA. |
| Confidence score: |
0.7032 |