circRNA basic information
circBase ID: hsa_circ_0001445
Name: hsa_circ_SMARCA5
Synonym: circSMARCA5
Host Gene: SMARCA5
Genomic location(hg19): chr4:144464661-144465125:+
Genomic location(hg38): chr4:143543508-143543972:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HOS; MG-63; OB3
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TXNRD2, OPTN, RBM5, TUBB4B, BPNT1, LRPPRC, CENPA, DNAJD6, ADAR, RNF103, HIPK3, RAB32, TBR1, ADSS, ADAMTS13, LILRB2, WDFY2, ARL4C, ARPP19, B3GALT5, ZNF730, STX6, RCAN2, CDKN1B, LPCAT3
Associated microRNA: miR-17-3p, miR-432-5p, miR-561-3p, miR-10b-3p, miR-181c-3p, microRNA-432, miR-181c
Biological function: promotes osteosarcoma cell proliferation, adhesion, migration, and invasion; affects cell cycle progression
Molecular mechanism: circSMARCA5-mediated ceRNA network; predicted sponging of multiple miRNAs and associated mRNAs (CircNet in silico analysis)
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (other); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; Bioinformatics Analysis
Clinical significance:

circSMARCA5 could be indicated as a biomarker for OS
Description:

circSMARCA5 (hsa_circ_0001445) is up-regulated in osteosarcoma cells and functions as an oncogenic circRNA, promoting proliferation, adhesion, migration, and invasion. Mechanistically, the study proposes a CircNet-predicted ceRNA network in which circSMARCA5 sponges multiple miRNAs (e.g., miR-432-5p and miR-181c-3p) and connects to a set of downstream mRNAs, but the miRNA/mRNA interactions were not experimentally validated here.
Confidence score:

0.4546
Other information
Title:

circSMARCA5 Promoted Osteosarcoma Cell Proliferation, Adhesion, Migration, and Invasion through a Competing Endogenous RNA Network.

Journal: BioMed research international
Published: 2020
PubMed ID: 33029498
Study type:

biological research
Data availability: All raw data can be made available upon request.
Code availability: -