circRNA basic information
circBase ID: hsa_circ_0076305
Name: -
Synonym: circular RNA_0076305
Host Gene: -
Genomic location(hg19): chr6:41704448-41712252:-
Genomic location(hg38): chr6:41736710-41744514:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

PTX-sensitive NSCLC tissues; PTX-resistant NSCLC tissues; xenograft tumors

Cell lines:

A549; H1299; A549/PTX; H1299/PTX; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: TMPRSS4
Associated microRNA: miR-936
Biological function: Circ_0076305 contributes to paclitaxel resistance, proliferation, migration, invasion, and glycolysis, and suppresses apoptosis in NSCLC cells; circ_0076305 knockdown increases PTX sensitivity and exerts anti-tumor effects.
Molecular mechanism: Circ_0076305 acts as a ceRNA/miRNA sponge for miR-936, thereby up-regulating TMPRSS4 expression and promoting paclitaxel resistance and malignant phenotypes in NSCLC cells.
Biological pathway or process:

chemoresistance (promotes); drug resistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Clinical Sample Validation; In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

Circ_0076305 knockdown may provide a new strategy to overcome the PTX resistance of NSCLC patients.

Description:

Circ_0076305 is up-regulated in paclitaxel-resistant NSCLC tissues and cells. It promotes PTX resistance and malignant cellular behaviors by sponging miR-936 to increase TMPRSS4 expression, while its knockdown enhances PTX sensitivity, suppresses proliferation, migration, invasion and glycolysis, and promotes apoptosis.

Confidence score:

0.7552

Other information
Title:

Knockdown of circ_0076305 decreases the paclitaxel resistance of non-small cell lung cancer cells by regulating TMPRSS4 via miR-936.

Journal: Toxicology research
Published: 2024
PubMed ID: 38993483
Study type:

combined biological and clinical study

Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -