| Expression pattern: |
UP |
| Associated gene: |
Junctional adhesion molecule 3 (JAM3), Ago2 |
| Associated microRNA: |
miR-127 |
| Biological function: |
Promotes ovarian cancer progression by enhancing proliferation, invasion/migration, tumor growth and lung metastasis. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-127 to upregulate JAM3; circKIF4A and JAM3 are recruited to an Ago2-related RISC and compete for miR-127 binding. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; BrdU; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circKIF4A could be a novel biomarker and therapeutic target for ovarian cancer. |
| Description: |
circKIF4A is up-regulated in ovarian cancer tissues and promotes malignant phenotypes (proliferation, invasion/migration, tumor growth and lung metastasis). Mechanistically, it mainly localizes in the cytoplasm and acts as a ceRNA to sponge miR-127, thereby increasing JAM3 expression via an Ago2/RISC-associated interaction. |
| Confidence score: |
0.7759 |