circRNA basic information
circBase ID: hsa_circ_0005785
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr12:110819556-110834257:-
Genomic location(hg38): chr12:110381751-110396452:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; paired adjacent normal tissues

Cell lines:

Huh7; Hep3B; SK-Hep1; HCCLM3; LO2

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: APRIL, Ago2
Associated microRNA: miR-578
Biological function: promotes proliferation and metastasis; inhibits apoptosis; promotes cell-cycle progression; promotes tumor growth in vivo
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-578 to derepress APRIL expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis

Clinical significance:

hsa_circ_0005785 was related to HCC patients' TNM stage and overall survival; overexpression rendered HCC patients' poor overall survival time

Description:

In hepatocellular carcinoma, hsa_circ_0005785 is up-regulated and promotes malignant phenotypes (proliferation, migration/invasion/metastasis) while inhibiting apoptosis and relieving cell-cycle arrest. Mechanistically, it functions as a cytoplasmic ceRNA sponging miR-578 to derepress the miR-578 target APRIL, thereby driving tumor progression and associating with poor overall survival.

Confidence score:

0.8712

Other information
Title:

Upregulated hsa_circ_0005785 Facilitates Cell Growth and Metastasis of Hepatocellular Carcinoma Through the miR-578/APRIL Axis.

Journal: Frontiers in oncology
Published: 2020
PubMed ID: 32974140
Study type:

combined biological and clinical study

Data availability: https://figshare.com/s/6d9d372ccb7d638f6d65
Code availability: -