| Expression pattern: |
UP |
| Associated gene: |
TRAF6, p65, NF-kappaB pathway |
| Associated microRNA: |
miR-7 |
| Biological function: |
Promotes breast cancer cell migration, dissemination and metastasis; remodels tumour microenvironment by increasing pro-inflammatory chemokine production and recruiting neutrophils. |
| Molecular mechanism: |
circ-TPGS2 acts as a ceRNA sponge for miR-7, increases TRAF6, activates NF-kappaB signaling through p65 phosphorylation and nuclear translocation, and forms a positive feedback loop through p65-mediated transcriptional activation of circ-TPGS2. |
| Biological pathway or process: |
NF-kappaB (promotes); migration (promotes); metastasis (promotes); inflammation (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); circRIP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-TPGS2 expression was linked to poor overall and recurrence-free survival and could be used as a promising prognostic marker for breast cancer. |
| Description: |
circ-TPGS2 is up-regulated in metastatic breast cancer tissues and high-metastatic breast cancer cell lines and predicts poorer overall and recurrence-free survival. Functionally, it promotes breast cancer migration, dissemination and lung metastasis while remodeling the tumor microenvironment by inducing pro-inflammatory chemokines and neutrophil recruitment. Mechanistically, circ-TPGS2 sponges miR-7 to increase TRAF6 and activate NF-kappaB signaling, while p65 transcriptionally activates circ-TPGS2 to form a positive feedback loop. |
| Confidence score: |
0.8757 |