| Expression pattern: |
DN |
| Associated gene: |
AKT/mTOR pathway |
| Associated microRNA: |
miR-19a-3p |
| Biological function: |
Tumor-suppressive in cervical cancer: increased circHIAT1 contributes to inhibition of cell growth and cell cycle progression and promotion of apoptosis; required for baicalein-mediated suppression of CC and tumor growth in vivo. |
| Molecular mechanism: |
circHIAT1 acts as a miRNA sponge (ceRNA-like) for miR-19a-3p, contributing to AKT/mTOR pathway inactivation in baicalein-treated cervical cancer cells. |
| Biological pathway or process: |
PI3K/AKT/mTOR (inhibits); apoptosis (promotes); cell cycle (inhibits); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
CircHIAT1 is downregulated in CC tissues and is suggested as an available target in baicalein-mediated treatment of CC. |
| Description: |
circHIAT1 is downregulated in cervical cancer tissues/cells but is induced by baicalein. It suppresses CC malignant behaviors by sponging miR-19a-3p and contributing to AKT/mTOR pathway inactivation, thereby inhibiting proliferation/cell cycle progression and promoting apoptosis, including reduced xenograft tumor growth. |
| Confidence score: |
0.705 |