circRNA basic information
circBase ID: hsa_circ_0000291
Name: -
Synonym: circ_0000291
Host Gene: -
Genomic location(hg19): chr11:35163017-35163328:+
Genomic location(hg38): chr11:35141470-35141781:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Tumor tissues; adjacent normal tissues
Cell lines:

MCF-7; MDA-MB-468; MDA-MB-231; BT-20; BT-474; MCF-10A; HEK-293
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: ETS1
Associated microRNA: miR-326
Biological function: Promotes breast cancer cell proliferation, migration, invasion, metastasis and EMT; functions as an oncogenic circRNA in breast cancer.
Molecular mechanism: circ_0000291 acts as a sponge/decoy of miR-326, thereby upregulating ETS1 expression and promoting breast cancer progression via the circ_0000291/miR-326/ETS1 axis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; BrdU; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

High circ_0000291 expression was associated with increased tumor size, local lymph node metastasis, higher Ki67 expression and increased T stage in breast cancer patients; it was proposed as a potential therapy target and basis for BC diagnosis and treatment.
Description:

circ_0000291 is upregulated in breast cancer tissues and cell lines and is associated with more aggressive clinicopathological features. Functionally, it promotes BC cell proliferation, migration, invasion, metastasis and EMT. Mechanistically, circ_0000291 sponges miR-326 to increase ETS1 expression, forming a circ_0000291/miR-326/ETS1 oncogenic axis.
Confidence score:

0.7537
Other information
Title:

The circRNA circ_0000291 acts as a sponge of microRNA 326 to regulate E26 transformation-specific sequence-1 expression and promote breast cancer progression.

Journal: Pathology international
Published: 2020
PubMed ID: 32869935
Study type:

combined biological and clinical study
Data availability: The data used to support the findings of this study are available from the corresponding author upon request.
Code availability: -