circRNA basic information
circBase ID: hsa_circ_0104345
Name: hsa_circ_IQCH
Synonym: circIQCH
Host Gene: IQCH
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary breast cancer tissues; lung metastatic tissues; adjacent normal tissue; tumor tissues; lung
Cell lines:

MCF-10A; MDA-MB-451; HCC38; BT549; HCC1806; MCF-7; MDA-MB-231; T47D; BT474; SKBR-3; MDA-MB-468
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: DNMT3A, PTEN, BRCA1, Ago2
Associated microRNA: miR-145
Biological function: promotes breast cancer cell proliferation, tumor growth, migration and lung metastasis
Molecular mechanism: ceRNA mechanism: circIQCH sponges miR-145 to upregulate DNMT3A, and is associated with reduced PTEN and BRCA1 expression
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); metastasis (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation
Clinical significance:

circIQCH could be a potential therapeutic target for breast cancer.
Description:

circIQCH (hsa_circ_0104345), derived from IQCH, is upregulated in primary and metastatic breast cancer tissues and in breast cancer cell lines. Functionally, circIQCH promotes proliferation, migration and lung metastasis. Mechanistically, circIQCH acts as a ceRNA sponge for miR-145, thereby increasing DNMT3A (and associated decreases in PTEN/BRCA1), forming a circIQCH-miR-145-DNMT3A axis.
Confidence score:

0.84
Other information
Title:

circIQCH sponges miR-145 to promote breast cancer progression by upregulating DNMT3A expression.

Journal: Aging
Published: 2020
PubMed ID: 32756009
Study type:

combined biological and clinical study
Data availability: -
Code availability: -