circRNA basic information
circBase ID: hsa_circ_0000376
Name: -
Synonym: circ_0000376
Host Gene: -
Genomic location(hg19): chr12:11199618-11248400:-
Genomic location(hg38): chr12:11047019-11095801:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissues; tumors

Cell lines:

H522; H1975; BEAS-2B

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: NOVA2, Ago2
Associated microRNA: miR-1182
Biological function: Promotes glycolysis, cell viability, migration, invasion, and tumor growth in NSCLC (including hypoxia-induced NSCLC).
Molecular mechanism: Acts as a ceRNA by sponging miR-1182, relieving miR-1182-mediated repression of NOVA2.
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis

Clinical significance:

High circ_0000376 expression was related to the poor overall survival of NSCLC patients and may be a potential biomarker for NSCLC treatment.

Description:

circ_0000376 is up-regulated in NSCLC and is further induced by hypoxia. Functionally, it promotes glycolysis and malignant phenotypes (viability, migration, invasion) and supports tumor growth. Mechanistically, it acts as a ceRNA that sponges miR-1182 to increase NOVA2 expression, forming a circ_0000376/miR-1182/NOVA2 axis associated with poor overall survival.

Confidence score:

0.8162

Other information
Title:

Circ_0000376, a Novel circRNA, Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1182/NOVA2 Network.

Journal: Cancer management and research
Published: 2020
PubMed ID: 32922073
Study type:

combined biological and clinical study

Data availability: GSE112214
Code availability: -