| Expression pattern: |
DS |
| Associated gene: |
EGFR, RAF1, FOS, NR4A3, PIK3CD, PAK1, CDK1 |
| Associated microRNA: |
miR-7 |
| Biological function: |
Not physiologically expressed in colon cancer cells; stromal cell-enriched expression in tumor microenvironment; correlations with miR-7 target genes are confounded by cancer-to-stromal cell ratios rather than ceRNA activity. |
| Molecular mechanism: |
Proposed miR-7 sponge/ceRNA interpretation is challenged in colon cancer; observed correlations can be explained by differing cancer-to-stromal cell ratios; ciRS-7 knockout does not change FOS expression in HEK293T cells. |
| Biological pathway or process: |
ceRNA regulation (inhibits) |
| Detected method: |
Q
H
S
|
| Validation methods: |
ISH (In Situ Hybridization); RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
High tumor ciRS-7 expression may explain previously reported poor prognosis associations, potentially reflecting tumor-stroma ratio rather than cancer-cell expression. |
| Description: |
In colon cancer tissues, ciRS-7 is not expressed in cancer cells but is abundant in tumor stromal cells. The study argues that bulk-tissue correlations between ciRS-7 and putative miR-7 target genes can be driven by variable cancer-to-stromal cell ratios rather than ceRNA (miR-7 sponge) activity, supported by ciRS-7 knockout and reporter assays in HEK293T cells. |
| Confidence score: |
0.6103 |