| Expression pattern: |
UP |
| Associated gene: |
E2F5, BAG4, FMNL2 |
| Associated microRNA: |
miR-145 |
| Biological function: |
Promotes proliferation and invasion; inhibits apoptosis; promotes cell cycle progression in colorectal cancer cells. |
| Molecular mechanism: |
Acts as a miRNA sponge of miR-145, inhibiting miR-145 transcriptional activity and thereby up-regulating miR-145 target genes/proteins (E2F5, BAG4, FMNL2). |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Clinical Sample Validation |
| Clinical significance: |
High circ_001569 expression correlates with differentiation, TNM classification, T classification, N classification, distant metastasis, and poor differentiation in CRC tissues. |
| Description: |
circ_001569 (hsa_circ_001569) is up-regulated in colorectal cancer tissues and promotes CRC cell proliferation and invasion while inhibiting apoptosis and cell-cycle arrest. Mechanistically, it functions as a miR-145 sponge, reducing miR-145 activity and thereby increasing downstream targets E2F5, BAG4 and FMNL2; its high expression is associated with aggressive clinicopathologic features. |
| Confidence score: |
0.7177 |