circRNA basic information
circBase ID: hsa_circ_0001910
Name: hsa_circ_WWC3
Synonym: circWWC3
Host Gene: WWC3
Genomic location(hg19): chrX:10031484-10066619:+
Genomic location(hg38): chrX:10063444-10098579:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; mice tumor tissues; lung; liver
Cell lines:

MDA-MB-231; MCF-7; MDA-MB-453; BT-549
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EGFR, GRB2, PAK4, MAPK1, AKT1, ZEB1
Associated microRNA: miR-26b-3p, miR-660-3p
Biological function: promotes proliferation, migration, invasion, tumor growth and metastasis in breast cancer
Molecular mechanism: ZEB1 transcriptionally upregulates circWWC3; circWWC3 sponges miR-26b-3p and miR-660-3p to increase Ras signaling pathway oncogenes (EGFR, GRB2, PAK4, MAPK1, AKT1) protein expression
Biological pathway or process:

Ras signaling pathway (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

circWWC3 is highly expressed in breast cancer tissues and elevated expression indicates poor overall survival; positively associated with clinical stage
Description:

In breast cancer, circWWC3 (hsa_circ_0001910) is upregulated and mainly localized in the cytoplasm. It promotes proliferation, migration, invasion, and in vivo growth/metastasis by sponging miR-26b-3p and miR-660-3p, thereby activating the Ras signaling pathway (e.g., increasing EGFR/GRB2/PAK4/MAPK1/AKT1 protein levels). High circWWC3 expression is associated with advanced clinical stage and poor overall survival.
Confidence score:

0.8961
Other information
Title:

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Journal: Molecular therapy. Nucleic acids
Published: 2020
PubMed ID: 32916598
Study type:

combined biological and clinical study
Data availability: https://doi.org/10.1016/j.omtn.2020.08.015
Code availability: -