| Expression pattern: |
UP |
| Associated gene: |
Rab11a, LC3B-II, p62, Beclin-1, ZO-1, occludin, Bcl-xl, caspase-3, caspase-9 |
| Associated microRNA: |
miR-26a-5p |
| Biological function: |
Promotes high glucose/methylglyoxal-induced autophagy, inflammation, endothelial cell injury, apoptosis-related protein changes, retinal vascular leakage, pericyte loss, acellular vessels, and microvascular dysfunction in diabetic retinopathy. |
| Molecular mechanism: |
circMKLN1 acts as a ceRNA/molecular sponge for miR-26a-5p, thereby regulating Rab11a expression and Rab11a-mediated autophagic flux in retinal microvascular endothelial dysfunction. |
| Biological pathway or process: |
autophagy (promotes); inflammation (promotes); apoptosis (promotes); ERK (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; IF (Immunofluorescence); IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model |
| Clinical significance: |
circMKLN1 may be a biomarker or therapeutic target for diabetic retinopathy. |
| Description: |
circMKLN1 is up-regulated in diabetic retinopathy and in HG/MG-treated hRMECs. It promotes endothelial injury and retinal microvascular dysfunction by sponging miR-26a-5p to enhance Rab11a-mediated autophagic flux, and its inhibition ameliorates vascular leakage and acellular vessels in STZ-induced diabetic mice. |
| Confidence score: |
0.7846 |