circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: circPVT1
Host Gene: PVT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005411
MONDO name: gallbladder cancer
Disease details: gallbladder cancer
Disease DO ID:
 3121
Disease MeSH ID:
 -
Disease NCIt ID:
 C7481
Disease ICD11 ID:
 922082116
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GBC tissues; adjacent normal tissues; tumor tissues
Cell lines:

GBC-SD; EHGB-1; SGC-996; NOZ; HIBEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MCL-1, Ago2
Associated microRNA: miR-339-3p
Biological function: Promotes GBC cell proliferation, migration and invasion; inhibits apoptosis; promotes tumor growth in vivo.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-339-3p to regulate MCL-1 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis
Clinical significance:

Higher circPVT1 expression was correlated with lymph node metastasis, advanced TNM stage, and poor overall survival (OS) in patients with GBC; independent prognostic factor.
Description:

circPVT1 is up-regulated in gallbladder cancer and is associated with lymph node metastasis, advanced TNM stage, and poor overall survival. Functionally, circPVT1 promotes GBC cell proliferation, cell-cycle progression, migration and invasion, and inhibits apoptosis, and its knockdown suppresses xenograft tumor growth. Mechanistically, circPVT1 acts as a cytoplasmic miRNA sponge for miR-339-3p to increase MCL-1 expression (circPVT1/miR-339-3p/MCL-1 axis).
Confidence score:

0.8097
Other information
Title:

CircPVT1 promotes gallbladder cancer growth by sponging miR-339-3p and regulates MCL-1 expression.

Journal: Cell death discovery
Published: 2021
PubMed ID: 34312371
Study type:

combined biological and clinical study
Data availability: -
Code availability: -