circRNA basic information
circBase ID: hsa_circ_0001846
Name: hsa_circ_UBAP2
Synonym: circ_0001846
Host Gene: UBAP2
Genomic location(hg19): chr9:33944362-33956144:-
Genomic location(hg38): chr9:33944364-33956146:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005178
MONDO name: osteoarthritis
Disease details: Osteoarthritis
Disease DO ID:
 8398
Disease MeSH ID:
 D010003
Disease NCIt ID:
 C3293
Disease ICD11 ID:
 558562409
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

articular cartilage tissues / OA cartilage tissues / normal articular cartilage tissues
Cell lines:

CHON-001
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: WNT5B
Associated microRNA: miR-149-5p
Biological function: circ_0001846 participates in IL-1beta-induced chondrocyte cell damage; circ_0001846 knockdown reverses IL-1beta-mediated inhibition of cell viability, migration and invasion, and reduces apoptosis, inflammation and ECM degradation in CHON-001 cells.
Molecular mechanism: Exosome-mediated circ_0001846 modulates IL-1beta-induced chondrocyte cell damage by sponging miR-149-5p and regulating WNT5B expression through the miR-149-5p/WNT5B axis.
Biological pathway or process:

ceRNA regulation (promotes); apoptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); inflammation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; ELISA; Western Blot
Clinical significance:

-
Description:

circ_0001846 is up-regulated in osteoarthritis cartilage tissues and IL-1beta-treated CHON-001 chondrocytes, including in exosomes. Functionally, circ_0001846 promotes IL-1beta-induced chondrocyte injury by aggravating apoptosis, inflammation and ECM degradation while reducing viability, invasion and migration. Mechanistically, exosome-mediated circ_0001846 acts through the miR-149-5p/WNT5B axis, suggesting a potential therapeutic target for OA.
Confidence score:

0.7799
Other information
Title:

Exosome-mediated circ_0001846 participates in IL-1beta-induced chondrocyte cell damage by miR-149-5p-dependent regulation of WNT5B.

Journal: Clinical immunology (Orlando, Fla.)
Published: 2021
PubMed ID: 34536574
Study type:

combined biological and clinical study
Data availability: All data and materials that support the findings of this study are available on request from the corresponding author.
Code availability: -