| Expression pattern: |
UP |
| Associated gene: |
CXCL5, Ago2 |
| Associated microRNA: |
miR-363-3p |
| Biological function: |
Promotes proliferation, cell cycle progression, migration and invasion, and inhibits apoptosis in colorectal cancer cells; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA to sponge miR-363-3p, thereby relieving miR-363-3p-mediated suppression and promoting CXCL5 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); invasion (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
CircCTNNA1 may serve as a biomarker of CRC. |
| Description: |
circCTNNA1 is up-regulated in colorectal cancer tissues and cell lines and mainly localizes to the cytoplasm. It promotes CRC malignant phenotypes and tumor growth by acting as a ceRNA that sponges miR-363-3p, thereby increasing CXCL5 expression. |
| Confidence score: |
0.78 |