circRNA basic information
circBase ID: mmu_circ_0001052
Name: mmu_circ_Hipk3
Synonym: circHIPK3
Host Gene: Hipk3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005267
MONDO name: heart disorder
Disease details: cardiac fibrosis
Disease DO ID:
 114
Disease MeSH ID:
 D006331
Disease NCIt ID:
 C3079
Disease ICD11 ID:
 1512587470
Disease OMIM ID:
 -
Species: Mouse
Species details: Mus musculus
Tissue specimen:

-
Cell lines:

HEK-293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TGF-beta2, Smad2, Smad3
Associated microRNA: miR-152-3p
Biological function: promotes cardiac fibroblast proliferation, migration and phenotypic switching (fibroblast-to-myofibroblast transition) under hypoxia
Molecular mechanism: acts as an endogenous sponge of miR-152-3p to upregulate TGF-beta2 expression and downstream Smad2/Smad3 signaling
Biological pathway or process:

proliferation (promotes); migration (promotes); fibrosis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; EdU Staining; Wound Healing Assay; Cell Cycle Assay; IF (Immunofluorescence); Western Blot
Clinical significance:

-
Description:

In hypoxia-treated mouse cardiac fibroblasts, circHIPK3 is upregulated and promotes proliferation, migration, and fibroblast-to-myofibroblast phenotypic switching. It functions as a ceRNA by sponging miR-152-3p, thereby increasing TGF-beta2 expression and enhancing downstream fibrotic signaling.
Confidence score:

0.6737
Other information
Title:

CircHIPK3 regulates cardiac fibroblast proliferation, migration and phenotypic switching through the miR-152-3p/TGF-beta2 axis under hypoxia.

Journal: PeerJ
Published: 2020
PubMed ID: 32904464
Study type:

biological research
Data availability: Zenodo: Liu Weiwei, & Wang Yan. (2020, July 9). CircHIPK3 regulates cardiac fibroblast proliferation, migration and phenotypic switching through the miR-152-3p/TGF-beta2 axis under hypoxia. Zenodo.
Code availability: -