| Expression pattern: |
UP |
| Associated gene: |
BCL2 |
| Associated microRNA: |
miR-324-5p, miR-361-3p |
| Biological function: |
promotes glioblastoma cell proliferation and inhibits apoptosis; knockdown represses tumor growth in vivo |
| Molecular mechanism: |
circSERPINE2 acts as a miRNA sponge for miR-361-3p and miR-324-5p to upregulate BCL2 expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
high circSERPINE2 expression level was significantly associated with the poor overall prognosis of GM patients |
| Description: |
circSERPINE2 is upregulated in glioblastoma tissues and cell lines and is mainly localized in the cytoplasm. It promotes glioblastoma proliferation and inhibits apoptosis by sponging miR-361-3p and miR-324-5p, thereby increasing BCL2 expression, and high circSERPINE2 correlates with poor prognosis. |
| Confidence score: |
0.8632 |