| Expression pattern: |
UP |
| Associated gene: |
EZH2, SUZ12 |
| Associated microRNA: |
- |
| Biological function: |
In proliferative cells represses p15 and p21; in RAF1-induced senescence promotes transcriptional induction of INK4 genes and supports senescence-associated gene program. |
| Molecular mechanism: |
Interacts with PRC2 components (EZH2, SUZ12); modulates EZH2 recruitment and H3K27ac/H3K27me3 at INK4 locus to affect transcription. |
| Biological pathway or process: |
cell cycle (other); proliferation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Transfection; RNA-seq; Bioinformatics Analysis; RNA Pull-Down; Western Blot; ChIP / ChIP-seq |
| Clinical significance: |
- |
| Description: |
circANRIL e16-e5 is up-regulated in RAF1 oncogene-induced senescence and helps drive transcriptional activation of INK4 genes. Mechanistically, RNAs containing the e16-e5 back-spliced junction bind PRC2 components (EZH2/SUZ12) and influence EZH2 recruitment and H3K27 acetylation/methylation at the INK4 locus. |
| Confidence score: |
0.7549 |