| Expression pattern: |
UP |
| Associated gene: |
Ago2, HK2, MMP9, MMP2, HIF1A, p70S6K1 |
| Associated microRNA: |
miR-200b, miR-200c |
| Biological function: |
Promotes glycolysis, migration, invasion and xenograft tumor growth in breast cancer under hypoxia; its knockdown suppresses these phenotypes. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-200b and miR-200c, negatively regulating their levels; functional rescue with miR-200b/c inhibition reverses effects of circDENND4C knockdown. |
| Biological pathway or process: |
glycolysis (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); PI3K/AKT/mTOR (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
High expression of circDENND4C was associated with tumor node metastasis stage, lymph node metastasis and tumor size; high expression predicted poor outcomes of patients with breast cancer. |
| Description: |
circDENND4C is up-regulated in breast cancer (and further induced by hypoxia) and promotes hypoxia-associated glycolysis, migration and invasion. Mechanistically, circDENND4C functions as a sponge for miR-200b and miR-200c; inhibiting circDENND4C increases miR-200b/c and suppresses tumor cell aggressiveness and xenograft growth. |
| Confidence score: |
0.7369 |