| Expression pattern: |
UP |
| Associated gene: |
LTBP2, AGO2 |
| Associated microRNA: |
miR-942-5p |
| Biological function: |
promotes OSCC cell proliferation, migration, invasion and EMT |
| Molecular mechanism: |
ceRNA sponge of miR-942-5p to increase LTBP2 expression; activates PI3K/Akt/mTOR signaling phosphorylation and accelerates EMT |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); PI3K/AKT/mTOR (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; ROC Analysis; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
High expression of circEPSTI1 is associated with high T stage and advanced TNM stage and predicts poorer OS and PFS; ROC analysis indicates it discriminates OSCC from OSF and OSF from NBM |
| Description: |
circEPSTI1 is sequentially up-regulated from NBM to OSF to OSCC and promotes OSCC cell proliferation, migration and invasion. Mechanistically, it functions as a ceRNA to sponge miR-942-5p, thereby increasing LTBP2 expression, accelerating EMT and enhancing PI3K/Akt/mTOR pathway activation; high circEPSTI1 predicts poor OS and PFS. |
| Confidence score: |
0.8961 |