| Expression pattern: |
DN |
| Associated gene: |
AKT3, Ago2 |
| Associated microRNA: |
miR-150-5p |
| Biological function: |
Promotes cell proliferation and migration; knockdown inhibits proliferation and migration in vitro |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-150-5p to modulate AKT3 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Potential diagnostic capability for HSCR (AUC 0.86; sensitivity 77%; specificity 94%) |
| Description: |
cir-ZNF609 is down-regulated in HSCR colon/bowel tissues and its knockdown inhibits cell proliferation and migration in 293T and SH-SY5Y cells. Mechanistically, cir-ZNF609 mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-150-5p, thereby modulating AKT3 expression, suggesting diagnostic potential for HSCR. |
| Confidence score: |
0.8703 |