circRNA basic information
circBase ID: hsa_circ_0077417
Name: hsa_circ_FBXL4
Synonym: circFBXL4
Host Gene: FBXL4
Genomic location(hg19): chr6:99347143-99375698:-
Genomic location(hg38): chr6:98899267-98927822:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; nontumor tissues; popliteal lymph node tissue
Cell lines:

GES-1; AGS; BGC-823; MGC-803; SGC-7901; HEK293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: STAT1, Ago2
Associated microRNA: miR-146a-5p
Biological function: inhibits proliferation; inhibits migration; inhibits invasion; inhibits tumorigenesis
Molecular mechanism: circFBXL4 acts as a ceRNA sponge for miR-146a-5p to regulate STAT1 expression (and downstream FN1/CHD4 via STAT1 transcriptional regulation).
Biological pathway or process:

ceRNA regulation (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; FISH / smFISH; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

-
Description:

In gastric cancer models, circFBXL4 is downregulated by LSECtin and acts as a ceRNA sponge for miR-146a-5p, thereby relieving miR-146a-5p-mediated repression of STAT1. Through this circFBXL4/miR-146a-5p/STAT1 axis, circFBXL4 suppresses GC cell proliferation, migration and invasion and counteracts LSECtin-driven malignant behaviors.
Confidence score:

0.8207
Other information
Title:

Novel roles of LSECtin in gastric cancer cell adhesion, migration, invasion, and lymphatic metastasis.

Journal: Cell death & disease
Published: 2022
PubMed ID: 35821222
Study type:

combined biological and clinical study
Data availability: supplementary data
Code availability: -