| Expression pattern: |
UP |
| Associated gene: |
TGF-beta2, SMAD3, p-SMAD3, E-cadherin, vimentin |
| Associated microRNA: |
miR-1305 |
| Biological function: |
promotes CRC cell proliferation, migration, invasion and EMT; exosome-transmitted circCOG2 enhances malignant phenotypes in recipient CRC cells and promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circCOG2 sponges miR-1305 to activate TGF-beta2/SMAD3 signaling; circCOG2 is transmitted via exosomes between CRC subpopulations |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circCOG2 is correlated with poor prognosis; high circCOG2 expression had lower OS; diagnostic potential (AUC reported) and independent prognostic factor |
| Description: |
circCOG2 (hsa_circ_0016866) is up-regulated in colorectal cancer tissues/plasma/exosomes and mainly localizes to the cytoplasm. It promotes CRC proliferation, migration, invasion and EMT by sponging miR-1305 and activating the TGF-beta2/SMAD3 pathway, and can be transmitted via exosomes to enhance malignant phenotypes in recipient CRC cells; high expression predicts poor overall survival. |
| Confidence score: |
0.8769 |