| Expression pattern: |
DN |
| Associated gene: |
FKBP5 |
| Associated microRNA: |
miR-556-5p |
| Biological function: |
Suppresses gastric cancer progression; overexpression induces apoptosis and inhibits cell proliferation, tumor growth, migration and invasion. |
| Molecular mechanism: |
circ_0003356 functions as a miR-556-5p sponge to regulate FKBP5 expression through the circ_0003356/miR-556-5p/FKBP5 axis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circ_0003356 is a potential prognostic biomarker of human gastric cancer; lower tumor circ_0003356 levels are associated with poorer overall survival, metastasis, histological tumor grade and clinical stage. |
| Description: |
hsa_circ_0003356/circ_0003356 is down-regulated in gastric cancer tissues and cell lines and is associated with favorable prognosis when expressed at higher levels. Functionally, circ_0003356 suppresses GC progression by sponging miR-556-5p and increasing FKBP5, thereby inhibiting proliferation, migration, invasion and tumor growth while promoting apoptosis. |
| Confidence score: |
0.8823 |