| Expression pattern: |
UP |
| Associated gene: |
SERPINE1, Ago2, exosomes |
| Associated microRNA: |
miR-486-5p |
| Biological function: |
promotes cell growth, migration, invasion, EMT and tumorigenesis in GBM |
| Molecular mechanism: |
circZNF652 acts as a ceRNA to sponge miR-486-5p and upregulate SERPINE1; exported in GBM cell-derived exosomes |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (other); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High CircZNF652 expression is associated with advanced clinical stage and worse prognosis/poor survival in GBM patients. |
| Description: |
circZNF652 is upregulated in glioblastoma and promotes malignant phenotypes (proliferation, migration, invasion, EMT and tumorigenesis). Mechanistically, it functions as a ceRNA that sponges miR-486-5p to increase SERPINE1 expression, and high circZNF652 correlates with poor patient survival. |
| Confidence score: |
0.8595 |