| Expression pattern: |
UP |
| Associated gene: |
Wnt1, beta-catenin |
| Associated microRNA: |
miR-149 |
| Biological function: |
circRNA_0044516 promotes GC cell proliferation and tumor growth and inhibits apoptosis; its knockdown suppresses cell viability, promotes apoptosis, and attenuates xenograft tumor growth. |
| Molecular mechanism: |
circRNA_0044516 targets miR-149 and regulates the Wnt1/beta-catenin pathway through miR-149, thereby promoting GC cell proliferation. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circRNA_0044516 expression was associated with T stage in GC patients, with higher relative expression in T3/4 than T1/2 tumors. |
| Description: |
circRNA_0044516 is upregulated in gastric cancer tissues and cell lines and functions as an oncogenic circRNA. It targets miR-149 to regulate the Wnt1/beta-catenin pathway, thereby promoting GC cell viability/proliferation and suppressing apoptosis; knockdown also inhibits HGC-27 xenograft tumor growth in nude mice. |
| Confidence score: |
0.7032 |