| Expression pattern: |
UP |
| Associated gene: |
LRRK2, GLUT1, HK2 |
| Associated microRNA: |
miR-1208 |
| Biological function: |
Promotes NSCLC cell proliferation, invasion, tumor growth, and glycolysis, and inhibits apoptosis. |
| Molecular mechanism: |
circ_0004630 functions as a cytoplasmic ceRNA by sponging miR-1208, thereby upregulating LRRK2 expression and promoting malignant behaviors and glycolysis in NSCLC. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
High circ_0004630 expression is associated with poor prognosis in NSCLC patients and circ_0004630 may be a biomarker and therapeutic target. |
| Description: |
circ_0004630 is up-regulated in NSCLC tissues and cell lines, and high expression is associated with poorer survival. Functional knockdown experiments indicate that circ_0004630 promotes NSCLC proliferation, invasion, tumor growth, and glycolysis while suppressing apoptosis. Mechanistically, cytoplasmic circ_0004630 acts as a miR-1208 sponge to upregulate LRRK2. |
| Confidence score: |
0.8557 |