| Expression pattern: |
UP |
| Associated gene: |
HMGB3 |
| Associated microRNA: |
miR-145 |
| Biological function: |
promotes proliferation, colony formation, migration and invasion/metastasis of NSCLC cells; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circEPSTI1 sponges miR-145 to elevate HMGB3 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
High expression of circEPSTI1 was associated with low survival rate of NSCLC patients. |
| Description: |
circEPSTI1 is up-regulated in NSCLC tissues/cells and its high level correlates with worse survival. Functionally, circEPSTI1 promotes NSCLC proliferation and metastasis-related behaviors and enhances tumor growth in vivo by sponging miR-145 to derepress HMGB3. |
| Confidence score: |
0.7583 |