| Expression pattern: |
UP |
| Associated gene: |
sp1, myc, beta-catenin |
| Associated microRNA: |
miR-363 |
| Biological function: |
Promotes GC cell proliferation, migration, invasion, tumorigenesis and metastasis; inhibits apoptosis. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-363 to enhance sp1 expression; sp1 transcriptionally activates myc and potentiates Wnt/beta-catenin signaling. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Overexpression of circ0005654 was correlated with a poor long-term prognosis in patients with GC; ROC AUC=0.781 indicates diagnostic value. |
| Description: |
circ0005654 is up-regulated in gastric cancer tissues and cell lines and is mainly localized in the cytoplasm. It promotes malignant phenotypes (proliferation, migration/invasion, tumor growth and metastasis) by sponging miR-363 to increase sp1, which activates myc transcription and potentiates Wnt/beta-catenin signaling. High circ0005654 is associated with poor prognosis and has diagnostic value in GC. |
| Confidence score: |
0.7976 |