| Expression pattern: |
UP |
| Associated gene: |
SLC1A5, Ago2 |
| Associated microRNA: |
miR-107 |
| Biological function: |
promotes proliferation, invasion and glutamine metabolism; inhibits apoptosis in esophageal cancer cells |
| Molecular mechanism: |
ceRNA (miRNA sponge) mechanism: circ-SFMBT2 sponges miR-107 to upregulate SLC1A5 expression |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis |
| Clinical significance: |
high expression of circ-SFMBT2 is associated with decreased overall survival and may be a prognostic target for EC patients |
| Description: |
circ-SFMBT2 is up-regulated in esophageal cancer tissues/cells and mainly localizes in the cytoplasm. It promotes malignant phenotypes (proliferation, invasion, glutamine metabolism and in vivo tumor growth) by sponging miR-107 and thereby increasing SLC1A5 expression; high circ-SFMBT2 correlates with poorer overall survival. |
| Confidence score: |
0.828 |