| Expression pattern: |
UP |
| Associated gene: |
ROCK1, Ago2 |
| Associated microRNA: |
miR-1297 |
| Biological function: |
Promotes HCC cell proliferation, migration, invasion, and tumorigenesis. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-1297 to regulate ROCK1 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High circ_101141 expression was related with lower overall survival rate in HCC patients. |
| Description: |
circ_101141 (circular ANAPC7) is up-regulated in HCC tissues and cell lines and is associated with worse overall survival. Functionally, circ_101141 promotes proliferation, cell-cycle progression, migration/invasion, and in vivo xenograft tumor growth. Mechanistically, it acts as a ceRNA sponging miR-1297 to increase ROCK1 expression. |
| Confidence score: |
0.8457 |