circRNA basic information
circBase ID: hsa_circ_0041732
Name: hsa_circ_FAM64A
Synonym: circFAM64A
Host Gene: FAM64A
Genomic location(hg19): chr17:6350782-6351078:+
Genomic location(hg38): chr17:6447462-6447758:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

TNBC tissues; paracancerous tissues; adjacent normal tissues; normal breast tissues; tumor tissues
Cell lines:

MCF-10A; MDA-MB-231; MDA-MB-453
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: CDT1, Cdc10-dependent transcript 1
Associated microRNA: miR-149-5p
Biological function: CircFAM64A plays an oncogenic role in TNBC by enhancing cell proliferation, cell cycle progression, migration, and invasion.
Molecular mechanism: CircFAM64A functions as a ceRNA/molecular sponge for miR-149-5p, relieving miR-149-5p-mediated repression of CDT1 and increasing CDT1 expression.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

CircFAM64A could be used as a diagnostic biomarker and potential target for TNBC therapy.
Description:

CircFAM64A is up-regulated in TNBC tissues and cell lines and acts as an oncogenic circRNA. It promotes proliferation, cell cycle progression, migration, and invasion by sponging miR-149-5p and increasing CDT1 expression, suggesting potential value as a diagnostic biomarker and therapeutic target.
Confidence score:

0.8118
Other information
Title:

CircFAM64A enhances cellular processes in triple-negative breast cancer by targeting the miR-149-5p/CDT1 axis.

Journal: Environmental toxicology
Published: 2022
PubMed ID: 35048507
Study type:

combined biological and clinical study
Data availability: GSE101124; data available from the corresponding author upon reasonable request
Code availability: -