| Expression pattern: |
UP |
| Associated gene: |
SLC7A8, ZNF618, ELAVL1/HuR |
| Associated microRNA: |
miR-9-5p |
| Biological function: |
promotes cell proliferation and colony formation; inhibits doxorubicin-induced apoptosis; promotes migration and invasion; promotes tumor growth in vivo |
| Molecular mechanism: |
putative ceRNA sponging of miR-9-5p to regulate downstream genes (e.g., SLC7A8, ZNF618); circFLNB knockdown alters tumor-related gene expression programs |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (other); ceRNA regulation (other); chemoresistance (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Colony Formation Assay; Western Blot; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circFLNB is up-regulated in OSCC tumors and functions as a pro-tumorigenic circRNA that promotes proliferation, survival under doxorubicin stress, migration/invasion, and xenograft tumor growth. Mechanistically, the study proposes a ceRNA axis involving circFLNB and miR-9-5p with shared targeting of downstream genes such as SLC7A8 and ZNF618, supported by RNA-seq/co-expression and knockdown transcriptome profiling. |
| Confidence score: |
0.7634 |