circRNA basic information
circBase ID: hsa_circ_0000517
Name: hsa_circ_RPPH1
Synonym: -
Host Gene: RPPH1
Genomic location(hg19): chr14:20811404-20811492:-
Genomic location(hg38): chr14:20343245-20343333:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjoining normal tissues; xenograft tissues; tumor tissues

Cell lines:

THLE-2; HCCLM3; Huh7; MHCC97-H

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: SMAD6, Ago2
Associated microRNA: miR-326
Biological function: promotes cell proliferation, colony formation, migration and invasion; promotes cell cycle progression; promotes tumor growth in vivo
Molecular mechanism: Acts as a competing endogenous RNA (ceRNA) sponging miR-326 to regulate SMAD6 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); metastasis (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model

Clinical significance:

High hsa_circ_0000517 expression was associated with shorter survival time in HCC patients and correlated with tumor size, TNM state, and lymph node metastasis

Description:

In hepatocellular carcinoma, hsa_circ_0000517 is up-regulated and functions as an oncogenic circRNA by promoting proliferation, cell-cycle progression, migration/invasion, and xenograft tumor growth. Mechanistically, it acts as a ceRNA that sponges miR-326 to increase SMAD6, forming a hsa_circ_0000517/miR-326/SMAD6 regulatory axis associated with poor patient survival.

Confidence score:

0.8006

Other information
Title:

Circular RNA hsa_circ_0000517 modulates hepatocellular carcinoma advancement via the miR-326/SMAD6 axis.

Journal: Cancer cell international
Published: 2020
PubMed ID: 32774154
Study type:

combined biological and clinical study

Data availability: All data generated or analysed during this study are included in this published Article.
Code availability: -