| Expression pattern: |
UP |
| Associated gene: |
USP7, Cyclin A2 |
| Associated microRNA: |
miR-34a |
| Biological function: |
Promotes HCC growth/proliferation, migration and metastasis, and reduces DNA damage. |
| Molecular mechanism: |
Exosomal circ-DB functions as a miRNA sponge for miR-34a, leading to activation of USP7 and downstream Cyclin A2 deubiquitination signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); ubiquitination (inhibits); cell cycle (promotes); apoptosis (not specified); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Transfection; Luciferase Reporter Assay; Western Blot; RNA Pull-Down; EdU Staining; Transwell Assay; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Plasma exosomal circ-DB is upregulated in HCC patients with higher body fat ratio and correlates with miR-34a and USP7 expression. |
| Description: |
Exosomal circ-DB (hsa_circ_0025129, from TNFRSF1A) is upregulated in HCC patients with higher body fat ratio. It promotes HCC proliferation/migration/metastasis and reduces DNA damage by sponging miR-34a, thereby increasing USP7 and activating the USP7/Cyclin A2 deubiquitination signaling axis. |
| Confidence score: |
0.7756 |