circRNA basic information
circBase ID: hsa_circ_0002211
Name: hsa_circ_DDX17
Synonym: circDDX17
Host Gene: DDX17
Genomic location(hg19): chr22:38890634-38897285:-
Genomic location(hg38): chr22:38494629-38501280:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; adjacent normal mucosa tissues

Cell lines:

SW480; SW620; HT29; LoVo; HCT116; RKO

In vivo animal model:

-

circRNA-disease information
Expression pattern:
DN
Associated gene: -
Associated microRNA: hsa-miR-21-5p
Biological function: Tumor suppressor; silencing promotes proliferation, migration and invasion and inhibits apoptosis in CRC cells
Molecular mechanism: Predicted miRNA sponge binding to hsa-miR-21-5p; downstream targets not experimentally validated
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other)

Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; divergent primers PCR; RNase R Treatment; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Bioinformatics Analysis

Clinical significance:

Decreased expression is associated with unfavorable clinicopathological parameters (lymphovascular invasion, depth of invasion, lymph node metastasis, distant metastasis, advanced TNM stage); potential biomarker and therapeutic target

Description:

circDDX17 (hsa_circ_0002211), derived from the DDX17 gene, is significantly down-regulated in colorectal cancer tissues and is associated with aggressive clinicopathological features. Functional assays show that circDDX17 suppresses CRC cell proliferation, migration and invasion while promoting apoptosis. Mechanistically, it is predicted to sponge hsa-miR-21-5p, but downstream targets were not experimentally confirmed.

Confidence score:

0.657

Other information
Title:

RNA sequencing reveals the expression profiles of circRNA and indicates that circDDX17 acts as a tumor suppressor in colorectal cancer.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2018
PubMed ID: 30591054
Study type:

combined biological and clinical study

Data availability: 10.1186/s13046-018-1006-x (supplementary material: Additional file 1-6)
Code availability: -